Hepatitis E Virus, ORF2, aa633-659, Recombinant (HEV)

Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function.\n\nThe protein contains immunodominant HEV ORF2 fragment, amino acids: (633-659).\n\nSpecificity:\nImmunoreactive with sera of HEV-infected individuals\n\nApplications:\nRecombinant HEV Antigen may be used in ELISA and Western Blot, excellent for detection of HEV with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H1927-28F

Size

100ug

Form

Supplied as a liquid in 25mM Tris-HCl, 1mM EDTA, 0.5M urea, 50% glycerol.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. The ORF2 protein of hepatitis E virus binds the 5' region of viral RNA. Surjit M, Jameel S, J Virol 2004 Jan;78(1):320-8 2. [Purification and immunological characterization of hepatitis E virus recombinant chimeric antigen encoded by ORF2 fragments and ORF3] Tang H, Ma YB, Le GY, Sun MS, Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2001 Au g;23(4):382-5 3. [Preliminary evidence that a hepatitis E virus (HEV) ORF2 recombinant protein protects cynomolgus macaques against challenge with wild-type HEV] Bi S, Lu J, Huang G, Shen X, Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2002 Mar;16(1):31-2 4. The full-length and N-terminal deletion of ORF2 protein of hepatitis E virus can dimerize. Tyagi S, Jameel S, Biochem Biophys Res Commun 2001 Aug 10;286(1):214-21 5. Augmentation of immune responses to hepatitis E virus ORF2 DNA vaccination by codelivery of cytokine genes. Tuteja R, Li TC, Jameel S, Viral Immunol 2000;13(2):169-78 6. Recombinant subunit ORF2.1 antigen and induction of antibody against immunodominant epitopes in the hepatitis E virus capsid protein. Li F, Riddell MA, Takeda N, J Med Virol 2000 Apr;60(4):379-86.