Hepatocyte Growth Factor (Hepatopoeitin-A, Scatter Factor, SF, HGF, HPTA)

Hepatocyte Growth Factor (HGF) is structurally similar, but not identical to scatter factor, a molecule shown to stimulate the dissociation and scattering of epithelial cells. It mapped to 7q21.1 and is involved in liver regeneration. Human HGF has been purified approximately 209,000-fold with 18% yield from plasma of a patient with fulminant hepatic failure. It consists of 2 chains, heavy and light with molecular weights of 54-65kD and 31.5-34.5kD, respectively. HGF is a potent mitogen, morphogen and motogen to both endothelial and epithelial cell types and is linked to a tyrosine kinase, proto-oncogene, c-met receptor. It indicated that HGF may serve as a paracrine mediator to control placental development and growth.\n\nApplications:\nSuitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunocytochemistry: 1ug/ml\nImmunohistochemistry (Frozen): 1ug/ml\nImmunohistochemistry (Paraffin): 1ug/ml. Heat mediated antigen retrival recommended.\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nCrossreactivity by Application:\nImmunocytochemistry: human\nImmunohistochemistry (Frozen): mouse and rat\nImmunohistochemistry (Paraffin): human, mouse and rat\nWestern Blot: human and rat\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts