HER2/ErbB2 Blocking Peptide

Supplier:

United States Biological

Cat no: H2031

HER2/ErbB2 Blocking Peptide

Peptide is used to block HER2/ErbB2 Rabbit mAb reactivity in immunohistochemistry protocols.\n\nThe ErbB2 (HER2) proto-oncogene encodes a transmembrane receptor-like glycoprotein of 185kD with intrinsic tyrosine kinase activity (1). ErbB2 does not have any known ligand. However, the kinase activity of ErbB2 can be activated without ligand if it is overexpressed and by heteromeric association with other members of the ErbB family (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. Direction of ErbB2 to the c-Cbl-regulated proteolytic pathway may have therapeutic potential. Tyr877 of ErbB2 is homologous to Tyr416 of pp60c-Src, located in the kinase domain. Phosphorylation of this site may be involved in regulation of ErbB2 biological activity. Tyr1248 and Tyr1221/1222 are the major autophosphorylation sites in ErbB2. Phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). \n\nQuality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks HER2/ErbB2 Rabbit mAb signal in immunohistochemistry.\n\nDrections for Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H2031

Size

100ug

Applications

IHC

Form

Supplied as a liquid in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol.

Purity

Highly Purified

References

(1) Muthuswamy, S.K. et al. (1999) Mol. Cell. Biol. 19, 6845

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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