Herpes Simplex 1, IgM (HSV1 IgM) BioAssay(TM) ELISA Kit

The United States Biological ELISA, HSV 1 IgM is intended for the detection of IgM antibodies\nto herpes simplex virus (HSV) 1.\n\nHerpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic.\nThere are two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with oral infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. United States Biological ELISA HSV IgM is an accurate serologic method to detect HSV specific antibody IgM in serum sample. Purified HSV antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the HSV 1 IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and TMB Chromogenic substrate is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.\n\nDetection Range Qualitative: Positive, Negative and Cut-off\nSample : 5ul \nSpecificity: 100%\nSensitivity: 100%\nTotal Time: 90min\nShelf Life: 12-18months\n\nKit Components: \nEach kit contains the following components in sufficient quantities to perform the number of tests indicated. All reactive reagents contain 0.1% sodium azide as a preservative \n\n1. Microtiter Plate\n2. Conjugate\n3. Calibrator\n4. Positive Control\n5. Negative Control\n6. TMB\n7. Wash Buffer, 10X Diluted wash buffer to 1X\n8. Sample Diluent\n9. Stop Solution \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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