HES1 (HL, HRY, hHL, bHLHb39, FLJ20408, HES-1, Hairy homolog, hairy homolog (Drosophila), Hairy-like protein, Hairy and enhancer of split 1, Class B basic helix-loop-helix protein 39)

Transcription factor HES-1, also called HRY or HES-1 is a protein that is encoded by the HES1 gene. This gene belongs to a family of basic helix-loop-helix (bHLH) proteins that are essential for neurogenesis, myogenesis, hematopoiesis, and sex determination. This gene is mapped to 3q29. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. This gene may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. It may play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.\n\nUniProt Number:\nQ14469\n\nGene ID:\nHES1\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144656

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Fukuda, A., Kawaguchi, Y., Furuyama, K., Kodama, S., Horiguchi, M., Kuhara, T., Koizumi, M., Boyer, D. F., Fujimoto, K., Doi, R., Kageyama, R., Wright, C. V. E., Chiba, T.Ectopic pancreas formation in Hes1-knockout mice reveals plasticity of endodermal progenitors of the gut, bile duct, and pancreas. J. Clin. Invest. 116: 1484-1493, 2006.\n2. Wendorff, A. A., Koch, U., Wunderlich, F. T., Wirth, S., Dubey, C., Bruning, J. C., MacDonald, H. R., Radtke, F. Hes1 is a critical but context-dependent mediator of canonical Notch signaling in lymphocyte development and transformation. Immunity 33: 671-684, 2010.

Additional Info

Recognizes human, mouse and rat HES1. No crossreactivity with other proteins.