Heterochromatin Protein 1, beta (HP1-beta, Chromobox Homolog 1, CBX1, Heterochromatin Protein p25 beta, HP1Hs-beta, M31, Modifier 1 Protein, MOD1, p25beta)

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified.\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other Applications not tested.\n\nRecommended Dilutions:\nELISA: 1:32,000\nImmunohistochemistry (paraffin): 2-3ug/ml\nWestern Blot: 0.3-1.0ug/ml\nOptimal dilutions to be determined by the researcher\n\nPositive Control:\nLysates of A431, HeLa and HepG2\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 monthsat -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See H2034-51G-P. Antibody

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SPECIFICATIONS

Catalog Number

H2034-51G

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

Sharma GG, Hwang KK, Pandita RK, Gupta A, Dhar S, Parenteau J, Agarwal M, Worman HJ, Wellinger RJ, Pandita TK. Human heterochromatin protein 1 isoforms HP1(Hsalpha) and HP1(Hsbeta) interfere \nwith hTERT-telomere interactions and correlate with changes in cell growth and response to ionizing radiation. Mol Cell Biol. 2003 Nov;23(22):8363-76.

Additional Info

Recognizes human CBX1 at ~25kD. Species sequence homology: mouse, rat, canine.