Hexokinase, Yeast, 140u/mg (HXK I)

Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P. Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate. At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized. Hexokinases (~100kDa for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins. The N-terminus, containing the mitochondrial target sequence, and the C-terminal has high sequence homology among various isoforms. The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus. HXK1 is located on chromosome 10. It exists either as a cytosolic protein or as a protein associated with the outer mitochondrial membrane via an interaction with porin, a voltage-dependent anion channel. Defects in HK1 gene causes hemolytic anemia.\n\nSpecific Activity: (same/more than) 140u/mg Protein (25 degrees C, pH 7.5)\n\nContaminants: (as HK activity = 100%) \nPhosphoglucose isomerase (same/less than) 0.1% \nGlutathione reductase (same/less than) 0.05% \nMyokinase (same/less than) 0.01% \nPhosphogluconate dehydrogenase (same/less than) 0.005% \nPhosphoglucomutase (same/less than) 0.01% \nGlucose-6-phosphate dehydrogenase (same/less than) 0.01% \nCreatine kinase (same/less than) 0.005% \nATPase (same/less than) 0.003%\n\nFormulation: \nOne ml of suspension contains\nProtein 2~10mg \nK-PO4 0.01~0.1mol/L \n(NH4)2SO4 3.0 mol/L \nEDTA.Na2 0.1~5.0mmol/L \npH 7.0

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