HIF1A (Hypoxia-inducible Factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting Protein, MOP1, PASD8)

Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1a is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1a is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1a degradation and lead to its stabilization. In addition, HIF-1a can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 8-21ug/ml\nImmunohistochemistry: 1:1000-1:8000\nImmunocytochemistry: 8-21ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136789

Size

25ug

Applications

ELISA, ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5, 437-48. 2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92, 5510-4. 3. Jaakkola P., et al. (2001) Science 292, 468-72. 4. Maxwell P.H., et al. (1999) Nature 399, 271-5. 5. Fukuda R., et al. (2002) J Biol Chem 277, 38205-11. 6. Jiang B.H., et al. (2001) Cell Growth Differ 12, 363- 9. 7. Laughner E., et al. (2001) Mol Cell Biol 21, 3995- 4004.

Additional Info

Recognizes human HIF1 alpha. Species Crossreactivity: Mouse, rat and bovine.

Alternative Names

Hypoxia-inducible Factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting Protein, MOP1, PASD8