HIF1AN (Hypoxia Inducible Factor 1 alpha Inhibitor, DKFZp762F1811, Factor Inhibiting HIF1, FIH1, FLJ20615, FLJ22027, Hypoxia Inducible Factor Asparagine Hydroxylase, Prolyl Hydroxylase)

FIH (Factor inhibiting HIF-1, HIF asparagine hydroxylase) is a dioxygen-dependent asparaginyl hydroxylase that modifies target protein function by hydroxylating target protein asparagine residues (1-3). Hypoxia-inducible factor (HIF), a transcriptional activator involved in control of cell cycle in response to hypoxic conditions, is an important target for FIH regulation. FIH functions as an oxygen sensor that regulates HIF function by hydroxylating at Asn803 in the carboxy-terminal transactivation domain (CAD) of HIF (4,5). During normoxia, FIH uses cellular oxygen to hydroxylate HIF-1 and prevent interaction of HIF-1 with transcriptional coactivators, including the CBP/p300-interacting transactivator. Under hypoxic conditions, FIH remains inactive and does not inhibit HIF, allowing the activator to regulate transcription of genes in response to low oxygen conditions (4-6). FIH activity is regulated in through interaction with proteins, including Siah-1, which targets FIH for proteasomal degradation (7). The Cut-like homeodomain protein CDP can bind the FIH promoter region to regulate FIH expression at the transcriptional level (8). Phosphorylation of HIF at Thr796 also can prevent FIH hydroxylation on Asn803 (9). Potential FIH substrates also include proteins with ankyrin repeat domains, such as Ik-B, Notch, and ASB4 (10-12). \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% \n Tween-20 at 4 degrees C with gentle shaking, overnight. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H3848-85H

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol and (same/less than)0.02% sodium azide.

P Type

Mab

Isotype

IgG

References

1. Koivunen, P. et al. (2004) J Biol Chem 279, 9899-904, 2. Linke, S. et al. (2004) J Biol Chem 279, 14391-7, 3. Lisy, K. and Peet, D.J. (2008) Cell Death Differ 15, 642-9, 4. Mahon, P.C. et al. (2001) Genes Dev 15, 2675-86, 5. Lando, D. et al. (2002) Genes Dev 16, 1466-71, 6. Lando, D. et al. (2002) Science 295, 858-61, 7. Fukuba, H. et al. (2007) Biochem Biophys Res Commun 353, 324-9, 8. Li, J. et al. (2007) Mol Cell Biol 27, 7345-53, 9. Lancaster, D.E. et al. (2004) Biochem J 383, 429-37, 10. Ferguson, J.E. et al. (2007) Mol Cell Biol 27, 6407-19, 11. Cockman, M.E. et al. (2006) Proc Natl Acad Sci USA 103, 14767-72, 12. Cockman, M.E. et al. (2009) Mol Cell Proteomics 8, 535-46.

Additional Info

Recognizes human FIH. Species crossreactivity: mouse, rat, monkey.