Hirudin, Recombinant

Recombinant Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech. Unlike heparin, Hirudin acts directly on thrombin rather than through other clotting factors.\n\nRecombinant Hirudin is derived from yeast and the polypeptide chain contains 65 amino acids, Mw is 6979.5 daltons. Recombinant Hirudin identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63. Recombinant Hirudin is purified by proprietary chromatographic techniques.\n\nBiological Activity: \nThe biological activity was measured using chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile dH2O. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H4080-05

Size

2ug

Form

Supplied as a lyophilized powder from 20mM PBS, pH 7.0, 2% mannitol. Reconstitute with sterile ddH2O to (same/more than)100ug/ml, which can then be further diluted to other aqueous solutions. For long term storage add 0.1% BSA or HSA.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. The use of PEG-Hirudin in chronic hemodialysis monitored by the Ecarin Clotting T1. The use of PEG-Hirudin in chronic hemodialysis monitored by the Ecarin Clotting Time: influence on clotting of the extracorporeal system and hemostatic parameters. Clin Nephrol 2006 Mar; 65(3):180-90 2. [Protective effects of hirudin on acute experimental intracerebral hemorrhage] Zhongguo Zhong Yao Za Zhi 2006 Jan;31(1):69-72 3. Pharmacokinetic study with N-Ile1-Thr2-63-desulfato-r-hirudin in rabbits by means of bioassay. J Zhejiang Univ Sci B 2006 Mar;7(3):241-4 4. Structure study of recombinant RGD-hirudin by vibrational and circular dichroism spectroscopy. Protein Pept Lett 2006 Jan;13(1):47-51 5. Role of Hirudin-like factor Va heavy chain sequences in prothrombinase function. J Biol Chem 2006 Mar 31;281(13):8773-9 6. Effect of hirudin versus heparin on hemocompatibility of blood contacting biomaterials: an in vitro study. Int J Artif Organs 2005 Dec;28(12):1272-7