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His-Tag (6-Histidine)

Cat no: H4951

His-Tag (6-Histidine)

A variety of plasmids contain DNA that encode an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography (1). As is the case with other protein tags (2), this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest (1).\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:25\niImmunofluorescence (IF/IC): 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H4951

Size

100ul

Applications

ICC, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography and immunoaffinity chromatography

Isotype

IgG

References

1. Kroll, D.J., et al., DNA Cell Biol. 12: 441-453 (1993). 2. di Guan, C., et al., Gene 67: 21-30 (1988).

Additional Info

Recognizes His-tag. Detects His-tagged recombinant proteins. Species Crossreactivity: all species

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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