His-Tag (6-Histidine) (HRP)

Insertion of a 6-histidine tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the His-tagged product. Such proteins are purified by immobilized metal ion afftinity chromatography which makes use of its high affinity for transition metal ion.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000\nELISA: 1:20-1:1000\nOptimal working dilutions to be determined by researcher.\n\nHybridoma: \nNS1 myeloma cells with spleen cells from Balb/c mice. \n\nSource: \nTissue cuture supernatant\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5099-16B

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, 0.01% thimerosal, HRP stabilizer. Labeled with horseradish peroxidase (HRP).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Conrath, K. et al. (2001) J. Biol. Chem. 276: 7346-7350. 2. Suen, J.L. et al. (2001) Immunol. 103: 301-309. 3. Hoffmann, S.C. et al. (2007) J. Biol. Chem. 282: 22370-22375. 4. Zheng, J. et al. (2007) BMC Immunol. 8: 4. 5. Bahi, A. & Dreyer, J.L. (2008) Genes Brain Behav. 7: 244-256. 6. Wrighton, K.H. et al. (2009) J. Biol. Chem. 284: 9755-9763.

Additional Info

Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Recognizes all His tagged proteins so far tested. May be used to track the purification of proteins expressed in bacteria, yeast or insect cells. In Western Blot of bacterial extracts, does not crossreact with any endogenous products. Some crossreactivity may be seen with extracts of insect or mammalian cells.