His-tag mAb

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.

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SPECIFICATIONS

Catalog Number

BS6501

Size

100ug/100ul

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Amo/Prot, Bac/Arc, Bov, Worm, Can, Chem, Ch/Bird, Dnk, Dro/Arth, ETag, Fel, Goat, GP, Ham, Horse, Moll, NHP, Plant, Prc, Rept, Rabbit

Form

liquid

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide His-tag.

Species

His-tag mAb detects over-expressed or recombinant proteins containing the his epitope tag.

Dilutions

WB: 1:2000~20000\nIF: 1:500~2000\nIP: 1:100

Swiss Prot

N/A

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul