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His-Tag Mouse mAb

His-Tag Mouse mAb

Cat no: MB2009

Supplier: Bioworld Technology, Inc.
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The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.
Catalogue number: MB2009
Reactivities: Human, Mouse, Rat, Amoeba/Protozoa, Bacteria/Archaea, Bovine, C. elegans/Worm, Canine, Chemical, Chicken/Bird, Donkey, Drosophila/Arthropod, Epitope Tag, Feline, Goat, Guinea Pig, Hamster, Horse, Mollusc, Non-Human Primate, Plant, Porcine, Reptile, Rabbit
Hosts: Mouse
Applications: Immunofluorescence, Western Blot
Size: 100ug/100ul
Swiss prot: N/A
Dilutions: WB: 1:5000~1:10000 IF: 1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide His-Tag.
Species: His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6His -tagged proteins.
Molecular weight: N/A
Purity: Affinity-chromatography
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Additional info: For research use only, not for use in diagnostic procedure.

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