Histone Deacetylase 6 (HDAC 6)

Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1). Eight members of HDAC family have been identified in the past several years (2,3). These HDAC family members are divided into two classes, I and II. Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3). These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo. \n\nApplications: \nSuitable for the detection of HDAC6 in human and mouse by Western blot. A protein of 134kD is detected in NIH-3T3 cells. Western blot analysis of HDAC6 in NIH-3T3 cell lysate with antibody. \n\nRecommended Dilution: \nWestern blot: 2ug/ml\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H5109-51A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Meinke PT and Liberator P. Curr Med Chem, 8(2):211-235 (2001). \n2. Nakayama T and Takami Y. J Biochem (Tokyo) 2001, 129 (4):491-499 (20001). \n3. Cress, W.D. and Seto, E. J. Cell. Physiol. 184, 1-16 (2000). \n

Additional Info

Can be used for detection of HDAC6 in human and mouse.