Histone Deacetylase, Assay Developer (2X) (HDAC)

Supplier:

United States Biological

Cat no: H5109-66B

Histone Deacetylase, Assay Developer (2X) (HDAC)

HDAC assay developer is an essential reagent for signal generation in HDAC or sirtuin assays done with any of HDAC fluorimetric substrates. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Typically, an 'inhibitor stop' will be included in the HDAC developer (e.g.Trichostatin A for class I and II HDACs, nicotinamide for sirtuins). \n\nApplications: \nStudy of HDAC or sirtuin kinetics, regulation and inhibitor sensitivity. Ideal for drug discovery and HTS applications. Cell-based HDAC assays. \n\nStorage and Stability: \n-70 degrees C. Store, long term, only as the 2X concentrate, at -70 degrees C. Concentrate is stable for at least 3 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid finger-rubbing for thawing). Frozen storage of smaller aliquots is recommended.

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SPECIFICATIONS

Catalog Number

H5109-66B

Size

6ml

Form

Supplied as a liquid (2X Concentrate).

References

1. K. Ito et al. Proc. Natl. Acad. Sci. USA 2002 99 8921\n2. B. Heltweg and M. Jung Anal. Biochem. 2002 302 175\n3. S. Milutinovic et al. J. Biol. Chem. 2002 277 20974

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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