Histone H1 (F1), Lysine Rich

Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.\nWith the exception of H1, the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly conserved (low mutation rate) proteins in nature. \n\nMolecular Weights of Histones:\nLysine Rich (H1, f1): ~ 31,500 \nSlightly Lysine Rich (H2a, f2a2): 14,004 \nSlightly Lysine Rich (H2b, f2b): 13,774 \nArginine Rich (H3, f3): 15,324 \nArginine Rich (H4, f2a1): 11,282\n\nDescription: \nHistone subclass F1\n\nSpecificity:\nSDS-PAGE Analysis: Doublet at ~31kD (GE Healthcare PhastGel Homogenous 20%)\n\nInstructions for Use: \nMost soluble in an acidic buffer such as 0.9M acetic acid at 1mg/ml. (Some assay systems may require more or less dilute histones.) Allow sufficient time for complete dissolution. Do not store diluted histones, as they will lose activity in solution. Prepare histone solution immediately before use.\n\nStorage and Stability:\nStore lyophilized histones desiccated at 4 degrees C. Reconstitute per reconstitution instructions. Do not store reconstituted histones. Histones lose activity in solutions. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H5110-07

Size

10mg

Form

Supplied as a white powder

Purity

Purified

References

1. Bradbury, E.M.: Histone Nomenclature in the Structure and Function of Chromatin, (Fitzsimmins, D.W., and Walstenholme, G.E.W., eds., American Elsevier, New York ) CIBA Foundation Symposium 28: 4 (1975). 2. Elgin, S.C.R. and Weinbrauh, H.: