Histone H1

The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependent manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30nm chromatin fiber. \n\nApplications:\nSuitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions:\nImmunofluorescence: Speckled staining is observed in the nucleus of normal and malignant cells.\nWestern Blot: 1:100. In blots of rat brain homogenate a duplet is observed at 27-29kD consistent with Histone H1. \nImmunohistochemistry (Frozen, paraffin): Acetone or PFA fixation of frozen sections is recommended.\nOptimal dilutions to be determined by the researcher. \n\nImmunohistochemistry Positive Control:\nTonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H5110-01E

Size

200ug

Applications

ELISA, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Swindle, C. S. & Engler, J. A. (1998) Association of the human papillomavirus type 11 E1 protein with histone H1. J. Virol. 72(3): 1994-2001. 2. Wang, X. et al. (2004) Histone H1-like protein participates in endothelial cell-specific activation of the von Willebrand factor promoter. Blood. 104(6): 1725-1732. 3. Hole, P.S. et al. (2010) Ras-induced reactive oxygen species promote growth factor-independent proliferation in human CD34+ hematopoietic progenitor cells. Blood. 115:1238-46.

Additional Info

Recognizes human Histone H1.