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Histone H2A, acidic patch, aa88-97

Cat no: H5110-08J

Histone H2A, acidic patch, aa88-97

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 -1:5000. Detects Histone H2A (acidic patch) in acid extracts from Hela cell lysates.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb \nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb \n\nPostive Control: \nHela cell extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5110-08J

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Ch/Bird, Yeast/Fun

Form

Supplied as a liquid in 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Wu, D., et al J Biol. chem 277:12001-8 (2002)

Additional Info

Recognizes human Histone H2A, at ~14kD. Species Crossreactivity: mouse, rat, chicken and yeast. Broad species crossreactiviy is expected.

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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