Histone H2B, acetylated (Lys12) (Histone H2B Type 1-B, Histone H2B.f, H2B/f, H2BFF, Histone H2B.1, H2B.1, Histone Cluster 1 H2bb, HIST1H2BB, MGC119804)

The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary\nbuilding block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1,2). Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, and 56), and H4 (Lys5, 8, 12, and 16), and is important for the regulation of histone deposition, transcriptional activation, DNA replication, recombination and DNA repair (1-3). Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the access of DNA to various DNA-binding proteins (4,5). In addition, acetylation\nof specific lysine residues creates docking sites for a protein module called the bromodomain, which binds to acetylated lysine residues (6). Many transcription and chromatin regulatory proteins contain bromodomains, and may be recruited to gene promoters, in part, through binding of acetylated histone tails. Histone acetylation is mediated by histone acetyltransferases (HATs), such as CBP/p300, GCN5L2, PCAF and Tip60, which are recruited to genes by DNA-bound protein factors to facilitate transcriptional activation (3). De-acetylation, which is mediated by histone deacetylases (HDAC and Sirtuin proteins), reverses the effects of acetylation and generally facilitates transcriptional repression (7,8).\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence (IF-IC): 1:100\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5110-09Z

Size

100ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Peterson, C.L. and Laniel, M.A. (2004) Curr Biol 14, R546-51. (2) Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9. (3) Roth, S.Y. et al. (2001) Annu Rev Biochem 70, 81-120. (4) Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79. (5) Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41. (6) Yang, X.J. (2004) Bioessays 26, 1076-87. (7) Haberland, M. et al. (2009) Nat Rev Genet 10, 32-42. (8) Haigis, M.C. and Sinclair, D.A. (2010) Annu Rev Pathol 5,253-95.

Additional Info

Recognizes endogenous levels of histone H2B, acetylated at Lys12. Species Crossreactivity: monkey, mouse, rat.

Alternative Names

Acetyl-Histone H2B