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Histone H2B, acetylated (Lys20) (Histone H2B Type 1, H2B-1A, H2B Histone Family, H2B2f, H2Ba, H2Bf, MGC131639)

Cat no: H5110-09D1

Histone H2B, acetylated (Lys20) (Histone H2B Type 1, H2B-1A, H2B Histone Family, H2B2f, H2Ba, H2Bf, MGC131639)

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) 2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5). \n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry/Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10 \nWestern Blot: 1:50,000 \nImmunoprecipitation: 1:10 \nImmunohistochemistry: 1:250-1:500 \nImmunocytochemistry/Immunofluorescence: 1:100-1:250 1:250-1:500 \nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nC6 cell lysate treated or untreated with TSA; Human cervical carcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

H5110-09D1

Size

100ul

Applications

FC, ICC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.01% sodium azide, \n50% glycerol.

P Type

Mab

Purity

Supernatant.

Isotype

IgG

References

1. Braunstein M, et al. Mol. Cell. Biol. 16:4349

Additional Info

Recognizes human Histone H2B acetylated on Lys 20. Species Crossreactivity: Mouse and rat.

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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