Histone H2B

Changes in chromatin structure play a key role in the regulation of transcription in eukaryotes. The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4. Acetylation of core histones regulates gene expression. Histone H2B is acetylated in the N-terminal region, primary at lysines 5, 12, 15, and 20. Ubiquitylation of histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3. Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of cell apoptosis and meiosis.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Paraformaldehyde treated sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5110-09A

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid. No preservative added.

P Type

Pab

Purity

Serum

Isotype

IgG

Additional Info

Reacts with histone H2B in human nuclear fractions. Does not crossreact with other histones, H1, H2A, H3 or H4. Species Crossreactivity: Binding of histone H2B from other species has not been tested. Species crossreactivity is expected due to sequence homology of histone H2B appears to be identical in all species examined.