Histone H3, acetylated (Lys9), phosphorylated (Ser10)

Applications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H5110-24M

Size

50ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

0.01M PBS

P Type

Pab

Purity

Purified

Isotype

IgG

References

Strahl BD & Allis CD The language of covalent histone modifications. Nature 403:41-5 (2000). <PUBMED:10638745>Cheung P et al. Signaling to chromatin through histone modifications. Cell 103:263-71 (2000). <PUBMED:11057899>Kornberg RD & Lorch Y Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome. Cell 98:285-94 (1999). <PUBMED:10458604>Thomson S et al. MAP kinase-mediated signalling to nucleosomes and immediate-early gene induction. Semin Cell Dev Biol 10:205-14 (1999). <PUBMED:10441074>Chadee DN et al. Increased Ser-10 phosphorylation of histone H3 in mitogen-stimulated and oncogene-transformed mouse fibroblasts. J Biol Chem 274:24914-20 (1999). <PUBMED:10455166>Sassone-Corsi P et al. Requirement of Rsk-2 for epidermal growth factor-activated phosphorylation of histone H3. Science 285:886-91 (1999). <PUBMED:10436156>Thomson S et al. The nucleosomal response associated with immediate-early gene induction is mediated via alternative MAP kinase cascades: MSK1 as a potential histone H3/HMG-14 kinase. EMBO J 18:4779-93 (1999). <PUBMED:10469656>De Cesare D et al. Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene. Proc Natl Acad Sci U S A 95:12202-7 (1998). <PUBMED:9770464>Struhl K Histone acetylation and transcriptional regulatory mechanisms. Genes Dev 12:599-606 (1998). <PUBMED:9499396>Zhang W et al. Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferase. EMBO J 17:3155-67 (1998). <PUBMED:9606197>Kuo MH et al. Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines. Nature 383:269-72 (1996). <PUBMED:8805705>Sobel RE et al. Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4. Proc Natl Acad Sci U S A 92:1237-41 (1995). <PUBMED:7862667>Barratt MJ et al. Mitogen-stimulated phosphorylation of histone H3 is targeted to a small hyperacetylation-sensitive fraction. Proc Natl Acad Sci U S A 91:4781-5 (1994). <PUBMED:8197135>Mahadevan LC et al. Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors. Cell 65:775-83 (1991). <PUBMED:2040014>

Additional Info

Staining of (acetyl K9) & (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse, Rat, Xenopus laevis, bovine, a wide range of other species, C. elegans, Chicken and Drosophilia melanogaster due to very high or perfect sequence identity.Not yet tested in other species.

Alternative Names

H3, HisH3, His1H3, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l