Histone H3

The relatively unstructured and highly charged N-terminal tail domains of histones are central to the processes that modulate chromatin structure. A diverse and elaborate array of post-translational modifications, including acetylation, phosphorylation, and methylation occurs on the N-terminal tail domains of histones, particularly of H3 and H4.1, 2 These modifications may alter chromatin structure and recruit downstream chromatin-associated proteins involved in transcription regulation. These in turn may dictate dynamic transitions between transcriptionally active or silent chromatin states.\nHistones H3 and H4 are the predominant histones modified by methylation and are highly methylated in mammalian cells.3, 4 Histone methylation, like acetylation, is a complex, dynamic process involving a number of processes, including transcriptional regulation, chromatin condensation, mitosis, and heterochromatin assembly. Moreover, lysine residues can be mono-, di-, and tri-methylated, adding further complexity to the regulation of chromatin structure.\nConserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 and Lys27 are the preferred sites of methylation.1, 4-6 Methylation of H3 at Lys9 is a modification intrinsically linked to epigenetic silencing and heterochromatin assembly. Histone H3 is methylated at Lys9 by site-specific H3 methyltransferases (HMTases) encoded by the SUV39H1 gene family.7 Methylation of H3 at Lys9 by SUV39H1 generates a binding site for HP1 proteins, a family of heterochromatic adaptor proteins implicated in both gene silencing and in the organization of higher order chromatin.8-11 Methylation of Lys9 interferes with the phosphorylation of Ser10 but is also influenced by preexisting modifications in the N-terminus of H3, such as H3 Ser10 phosphorylation itself.7 Conversely, in vivo deregulated SUV39H1 or disrupted SUV39H1activity modulates H3 Ser10 phosphorylation in native chromatin leading to aberrant mitotic divisions.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H5110-12R1

Size

50ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

0.01 PBS, pH 7.4 containing 1.0% BSA

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Strahl, B.D., and Allis, C.D., Nature, 403, 41-45(2000).\n2. Cheung, P., et al., Cell, 103, 263-271 (2000).\n3. Strahl, B.D., et al., Proc. Natl. Acad. Sci. USA, 96, 14967-14972 (1999).\n4. Rice, J.C., and Allis, C.D., Curr. Opin. Cell Biol., 13, 263-273 (2001).\n5. Jenuwien, T., and Allis, C.D., Science, 293, 1074-1080 (2001).\n6. Bird, A., Science, 294, 2113-2115 (2001).\n7. Rea, S., et al., Nature, 406, 593-599 (2000).\n8. Lachner, M., et al., Nature, 410, 116-120 (2001).\n9. Bannister, A.J., et al., Nature, 410, 120-124 (2001).\n10. Nakayama, J-I., et al., Science, 292, 110-113 (2001).\n11. Noma, K-I., et al., Science, 293, 1150-1155 (2001).

Additional Info

Detects a band at approximately 17kD. This Histone H3 sequences is identical in many species including mouse, rat, bovine, chicken, frog, drosophila and C. elegans. There is no inhibition with the non-methylated Histone H3 peptide. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with mouse, rat, bovine, chicken, frog, drosophila and C. elegans.

Alternative Names

H3, HisH3, His1H3, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l