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Histone H3 (Mono-Methyl K4) pAb

Histone H3 (Mono-Methyl K4) pAb

Cat no: BS7230

Supplier: Bioworld Technology, Inc.
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Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56.
Catalogue number: BS7230
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug/100ul
Swiss prot: P68431
Dilutions: WB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200 ChIP: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, mono methylated at K4.
Species: Histone H3 (Mono-Methyl K4) pAb detects endogenous levels of histone H3 only when mono-methylated at Lys4.
Molecular weight: ~ 17 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: H3K4me1
Additional info: For research use only, not for use in diagnostic procedure.

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