Histone H3, Monomethyl, Dimethyl, Trimethyl (Lys4) (Histone H3.1, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, H

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone methylation is primarily associated with heterochromatin and gene silencing.\n\nApplications:\nSuitable for use in Western Blot and Cell Function Assay. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:8000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRecombinant Histone H3 and HeLa acid extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029498

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 40% glycerol.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Zager, R.A. and Johnson, A.C. (2010). Am J Physiol Renal Physiol. 298(3):F827-37. 2. Benevolenskaya, E.V. (2007). Biochem Cell Biol. 85(4):435-43. 3. Strahl, B.D. (1999). Proc Natl Acad Sci USA. 96(26):14967-72.

Additional Info

Recognizes human Histone H3 mono/di/tri-methylated at Lys4.