Histone H3, phosphorylated (Ser10)

Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. \n\nApplications: \nSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:1000 \nImmunoprecipitation: 1:50 \nImmunohistochemistry (Paraffin): 1:200 Antigen retrieval is recommended using heating in citrate buffer.\nImmunohistochemistry (Frozen): 1:400 using 10% Neutral buffered formalin as a fixative\nFlow Cytometry: 1:50 \nImmunofluorescence (IF-IC): 1:100\nOptimal dilutions to be determined by the researcher.\nNote: Adherence to the attached protocol is extremely important.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5110-13J

Size

100ul

Applications

ELISA, FC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Ch/Bird, Dro/Arth, NHP, Yeast/Fun

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Workman, J.L. and Kingston, R.E., 1998, Annu. Rev. Biochem. 67, 545-579. 2. Hansen, J.C., et al., 1998, Biochemistry 37, 17637-17641. 3. Strahl, B.D. and Allis, C.D., 2000, Nature 403, 41-45. 4). 4.

Additional Info

Recognizes endogenous human histone H3 only when phosphorylated at serine 10. Does not crossreact with other phosphorylated histones or with acetylated histones. Species Crossreactivity: mouse, rat, monkey, chicken, D. melanogaster, S.cerevisiae and zebrafish. Species Sequence Homology: Xenopus