Histone H3 (Tri-Methyl K4) mAb

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms.

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SPECIFICATIONS

Catalog Number

MB2028

Size

100ug/100ul

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H3, Tri methylated at K4.

Species

Histone H3 (Tri-Methyl K4) mAb detects endogenous levels of histone H3 only when Tri-methylated at Lys4.

Dilutions

WB: 1:500~1:2000

Swiss Prot

P68431

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul