Kit Components:
H5110-15J1: Histone H4, acetylated (Lys5) 1x25ul
H5110-15J2: Histone H4, acetylated (Lys8) 1x20ul
H5110-15J3: Histone H4, acetylated (Lys12) 1x25ug
H5110-15J4: Histone H4, acetylated (Lys16) 1x20ul
Quality Control Testing
Immunoblot Analysis: Histone H4, acetylated antibodies detected increased levels of acetylated histone H4 in acid extracts of HeLa cells treated with 5mM sodium butyrate.
Resolved by electrophoresis, transferred to nitrocellulose and probed with a Histone H4, acetylated antibody, varying dilutions. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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