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HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1)

Cat no: H6003-34H

HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1)

HIV-1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Neutralization, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nELISA: Yields titers of >1:2000 with 100ng of immobilized gp120 under standard ELISA conditions. \nWestern Blot: 1-2ug/ml depending on the nature and quantity of the immobilized antigen. \nImmunohistochemistry: 1:200\nOptimal dilutions to be determined by the researcher. \n\nBiological Activity: \nNeutralizes HIV-1 as determined by syncycium assays using infected CD4 bearing cells and human PBL co-cultures.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H6003-34H

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography ((same/more than)95% SDS-PAGE, reduced)

Isotype

IgG

Additional Info

Recognizes native and recombinant HIV-1 gp120/gp160 in ELISA and Western Blot.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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