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HIV Integrase p31, Recombinant (Human Immunodeficiency Virus)

Cat no: H6003-15A

HIV Integrase p31, Recombinant (Human Immunodeficiency Virus)

ntegrase is an enzyme, a product of the HIV1 Gag/Pol gene; the other two enzymes being reverse transcriptase and protease. The human immunodeficiency virus (HIV-1) uses an enzyme, a so-called integrase, to carry out the integration of its viral DNA into the host chromosome thereby tricking the host cell machinery into making viral proteins. The HIV-1 integrase is a protein of 32kD and is composed of three domains. \n\nNo cellular homologue of HIV integrase has been described, so potential inhibitors to this enzyme could be relatively nontoxic.\n\nSource: \nRecombinant corresponding to HIV-1 immunodominant regions from integrase p31 (aa9-289) fused to 6-His-tag expressed in E. coli. \n\nApplications:\nSuitable for use in ELISA, Lateral Flow and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H6003-15A

Size

100ug

Applications

ELISA, WB

Form

Supplied as a liquid in 1.5M urea, 25mM Tris-HCl, pH 8.0, 0.02% Triton-X, 50% glycerol.

Purity

~95% (SDS-PAGE)

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