HL60 (HL-60, Leukemia Cell Line HL-60)

IPO-M6 can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia were IPO-M6 positive. The antigen, defined with this mAb, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages were also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B?ALL were IPO-M6 negative.\n\nApplications:\nSuitable for use in Immunohistochemistry, Flow Cytometry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10 \nImmunohistochemistry (Frozen): 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H6095

Size

100ug

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Sidorenko, S.P. et al. Monoclonal antibodies of the IPO series in studying and diagnosing malignant lymphoproliferative diseases. Gematol. Transfuziol. 35: 19-22 (1990). 2. Sidorenko, S.P. et al. Experem. Oncology 17: 242 (1995) (Russian).

Additional Info

Recognizes human leukemia cell line HL-60. Immunoprecipitates two proteins of MW of 48 and 52kD. Does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 or T-cell lines CCRF-HSB2, Jurkat and Molt-4.