HLA Class 1 Antigen A2 (PE)

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C.\nThe HLA A gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications have not been tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood.\nOptimal dilutions to be determined by researcher.\n\nRecommended Negative Control: \nI1904-84H3: IgG2b Negative Control (PE)\n\nHybridoma: \nNS1 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4 degrees C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H6098-07F

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Parham, P. and Brodsky, F.M., Hum. Immunol. 3: 277-299 (1981). 2. Hogan, K.T. and Brown, S.L., Hum. Immunol. 33: 185-192 (1992). 3. Harig, S., et al., Blood 98: 2999-3005 (2001). 4. Pantenburg, B. et al. (2010) Am J Trop Med Hyg. 82:600

Additional Info

Recognizes the human HLA-A2 histocompatibility antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.