HLA Class 1 Antigen ABC (Biotin)

Applications:\nSuitable for use in Flow Cytometry. Other applications have not been tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat-1:5. Use 20ul to label 10e6 cells or 100ul whole blood.\nOptimal working dilutions to be determined by researcher.\n\nRecommended Secondary Reagents:\nS7973-78: Streptavidin (PE)\nS7973-92F2: Streptavidin (PE)\nS7973-96A: Streptavidin (FITC) \nS7973-96B: Streptavidin (FITC)\n\nRecommended Negative Controls: \nI1904-83V1: IgG2a Negative Control (Biotin)\n\nHybridoma: \nSpleen cells from immunized BALB/c mice fused with mouse NS1/1-Ag4.1 myeloma cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H6098-39F2

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum, Bov, Fel, Moll, NHP

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, before the addition of glycerol to 40%. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Barnstable, C. J., et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14: 9 - 20. 2. Burrone, O. R. et al. (1982) Control of HLA-A, B, C sythesis and expression in interferon cells. EMBO J. 1; 345 - 349. 3. Jacobsen, C. N. et al. (1993) Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466. 4. Brodsky, F.M. et al. (1982). Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166. 5. Neefjes, J.J. et al. (1986). A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347. 6. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110. 7. Stern, P. et al. (1987). Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4): 1088 - 1091.

Additional Info

Recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. Recognizes virtually all nucleated human cells. Species Crossreactivity: Rhesus macaque, bovine, feline, Cynomolgus monkey and baboon.