HLA Class 1 Antigen ABC (FITC)

Human Leukocyte Antigen (HLA) class I which is also known as human class I Major Histocompatibility Complex (MHC). HLA class I is encoded by three HLA alleles: HLA-A, HLA-B and HLA-C. HLA class I is a protein dimer composed of an alpha chain (Mr =44kD) and a beta chain which is also known as b2 microglobulin (Mr =12kD). The smaller beta chain bears homology with immunoglobulin molecules. The alpha chain contains four distinct regions: a cytoplasmic region located at the carboxyl terminus of the protein, followed by a transmembrane region, an immunoglobulin-like region and a peptide-binding region located at the amino terminus. This peptide binding region contains a cleft which can bind peptides consisting of 9-11 amino acid residues. HLA class 1 specifically targets the bound peptide to T cells while interacting with the T-cell receptor. HLA class 1 appears to preferentially target CD8+ T cells, whereas HLA class II preferentially targets CD4+ T cells.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 10ul labels 10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

H6098-39C1

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 10mM sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Shaw, S., G.C. Luce, W.R. Gilks, K. Anderson et al. (1995) Leucocyte differentiation antigen database. In Leucocyte Typing V. Edited by Schlossman, S.F., L. Boumsell, W. Gilks, J.M. Harlan, T. Kishimoto, C. Morimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd. Oxford University Press p.190. 2. da Silva, A.J., M. Brickelmaier, G.R. Majeau, A.V. Lukashin, J. Peyman, A. Whitty, and P.S. Hochman (2002) Comparison of gene expression patterns induced by treatment of human umbilical vein endothelial cells with IFN-alpha 2b vs. IFN-beta 1a: Understanding the functional relationship between distinct type I interferons that act through a common receptor. Journal of Interferon and Cytokine Research 22(2):173-188. 3. Mhashilkar, A.M., C. Doebis, M. Seifert, A. Busch, C. Zani, J. Soo Hoo, M. Nagy, T. Ritter, H.-D. Volk, and W.A. Marasco (2002) Intrabody-mediated phenotypic knockout of major histocompatibility complex class I expression in human and monkey cell lines and in primary human keratinocytes. Gene Therapy :307-319.

Additional Info

Recognizes human Leukocyte Antigen (HLA) class I which is also known as human class I Major Histocompatibility Complex (MHC). Recognizes a determinant shared by HLA-A, HLA-B, and HLA-C alleles.