HLA Class 1 Antigen ABC

Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation.\n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry: Frozen\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.\n\nOptimal working dilutions to be determined by researcher.\n\nRecommended Positive Control:\nTonsil\n\nRecommended Negative Controls: \nMouse IgG2a Negative Control\n\nRecommended Secondary Reagents: \nF(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate\n\nFusion Partners: \nSpleen cells from immunized BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H6098-39F

Size

200ug

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Hum, Bov, Fel, Moll, NHP

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Barnstable, C. J., et al . (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14: 9 - 20/ \n2. Burrone, O. R. et al . (1982) Control of HLA-A, B, C sythesis and expression in interferon cells. EMBO J. 1; 345 - 349. \n3. Jacobsen, C. N. et al . (1993) Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466. \n4. Brodsky, F.M. et al . (1982). Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166. \n5. Neefjes, J.J. et al . (1986). A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347. \n6. Yoshino, N. et al . (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110 \n7. Stern, P. et al . (1987). Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4): 1088 - 1091.

Additional Info

Recognizes an antigenic determinant shared among products of the HLA A, B and C loci. The antibody recognizes virtually all nucleated human cells. Species Crossreactivity: Clone is reported to crossreact with Rhesus macaque (4), bovine (4), feline (5), Cynomolgus monkey (6) and baboon (7).