HLA Class 1 Antigen G (FITC)

HLA-G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells\n\nApplications: \nSuitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:500\nELISA: 1:2000\nImmunocytochemistry: 5ug/ml \nImmunohistochemistry (Frozen): 1:200\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

H6098-72M8

Size

100ug

Applications

ELISA, FC, ICC, IHC, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with FITC.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Menier C. et al. Hum Immunol 64; 315-326 (2003). 2. Pangault C. et al., Hum Immunol 63; 83-90 (2002). 3. Lozano J.M. et al., AIDS 16; 347-351 (2002). 4. Fuzzi B. et al., . Eur J Immunol 32; 311-315 (2002). 5. Fournel S. et al., Tissue Antigens 55; 510-518 (2000).

Additional Info

Recognizes the native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Does not react with mouse.