HLA Class 1 Granulocyte (FITC)

Supplier:

United States Biological

Cat no: H6098-70

HLA Class 1 Granulocyte (FITC)

Antibodies can also be used for the detection of HLA antigens on the lymphocytes in whole blood samples and cells isolated from blood or lymphoid tissue. The HLA Class 1 conjugated monoclonal antibodies can be used for the detection of specific cell surface antigens. \n\nApplications:\nSuitable for use in Flow Cytometry. Other potential applications include Cell Typing, Tissue Staining, and Chimerism Studies. Other applications have not been tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nFITC/Protein Ratio: \n4.0-8.0\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPlease contact United States Biological for bulk requirements and licensing requests relating to production applications.

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SPECIFICATIONS

Catalog Number

H6098-70

Size

250ul

Applications

FC, IF

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 0.5% BSA. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgM

References

1. NCCLS Tentative Standard. \"Leukocyte Differential Counting\" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. \"Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop\", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., Chen, T., Orpilla, J. and Lee, J.H. \"A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry\", Tissue Antigens 50:197-201 (1997).

Additional Info

Reacts to HLA Class 1 Granulocyte when used at a dilution of 1:10 when using the microcytoxicity test under standard NIH conditions.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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