HLA Class 2 Antigen DP

DP monomorphic antigen is expressed on hematopoietic progenitors, B lymphocytes, monocytes/ macrophages, dendritic cells, langerhans cells, activated T cells, some endothelial and epithelial cells, but not on resting T lymphocytes, erythrocytes and platelets. HLA-DP antigen is also expressed at high levels on B lymphocyte leukemic cells and cells of CML-LBC, at moderate levels on the cells of AML-M1/M2, M4 and M5, but not or little on the cells of AML-M3 and CML. This antibody might be used as valuable reagent in diagnosis and classification of leukemias.\n\nApplications:\nSuitable for use in indirect immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution: \nFlow Cytometry: 0.015ug/1x10e6 cells or 100ul peripheral blood.\nImmunohistochemistry: Acetone-fixed frozen sections and formalin-fixed paraffin sections.\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H6099-84E

Size

100ug

Applications

FC, IF, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.0, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Shen DC., Chen Z., Bai JF., et al., 1990. Identification and preliminary application of HI43- A monoclonal antibody against human HLA-DP monomorphic antigen. Chinese J. of Microbiology and Immunology. 10(5): 291. 2. Knapp, W., B. Dorken, E.P. Rieber, et al., eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens. P?28 Oxford University Press, New York.

Additional Info

Recognizes normal human peripheral blood lymphocytes (25%) and monocytes (95%). Does not recognize normal human periphral blood granlocytes.