HLA Class 2 Antigen DR (H2Ia)

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilution: \nFlow Cytometry: 1:50-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.\nImmunohistochemistry: frozen tissues\nImmunohistochemistry (paraffin): using PLP (periodate-lysine- paraformaldehyde) fixation\nOptimal dilutions to be determined by researcher. \n\nRecommended Negative Control: \nI1904-83M1: Rat IgG2a Negative Control \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H6099-97A

Size

250ug

Applications

FC, IHC

Hosts

Rat

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Brickell, P.M. et al. (1981) A monoclonal antibody to the HLA-DR product recognizes a polymorphic Ia determinant in mice. Immunology. 43: 493-501.\n2. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leucocytes in Paraffin-embedded Rat and Mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320. 3. Bellinghausen, I.et al. (2003) Production of interleukin-13 by human dendritic cells after stimulation with protein allergens is a key factor for induction of T helper 2 cytokines and is associated with activation of signal transducer and activator of transcription-6. Immunology. 108:167-176. 4. Choi, Y. et al. (2009) Immunohistochemical Characterization of the Human Sublingual Mucosa. Int. J. Oral. Biol. 34: 131-135.

Additional Info

Recognizes a monomorphic determinant of human HLA-DR, expressed by B lymphocytes, monocytes and activated T cells. Reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB (1).