HLA Class 2 Antigen DR (SLA Class II DR) (FITC)

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat; 10ul labels 10e6 cells in 100ul\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nX63-Ag.8.653 myeloma cells with spleen cells from Balb/c mice.\n\nRecommended Negative Control:\nI1904-84U3: IgG2b Negative Control (FITC)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H6099-95Z1

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Bov, Prc

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Bullido, R. et al. (1997) Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals. Dev. Comp. Immunol. 21:311-322. 2. Jeong, H.J. et al. (2010) Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus. Clin Vaccine Immunol. 17: 503-12.

Additional Info

Recognizes porcine HLA Class 2 Antigen DR (SLA-II DR) expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Reacts with lymphocytes from outbred and miniature pigs, suggesting a reaction with a monomorphic determinant. Immunoprecipitates a heterodimer composed of two polypeptides of 28 and 35kD from NP-40 extracts of biotin surface-labelled porcine peripheral blood mononuclear cells. Inhibits the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B. Species Crossreactivity: bovine