HLA Class 2 Antigen DR9 (X-Reactive)

Applications:\nThis antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.\n\nRecommended Dilution:\nCytotoxicity: 1:10. Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution. \nOptimal dilutions to be determined by the researcher.\n\nCytotoxicity:\nExpected Results:\nCell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.\nLimitations:\nCell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.\n\nSource:\nAscites fluid\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile buffer. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPlease contact United States Biological for bulk requirements and licensing requests relating to production applications.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H6100-35

Size

100ul

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, 1% BSA. Reconstitute to 100ul sterile dH2O to make a 10X stock solution.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

1. NCCLS Tentative Standard. \"Leukocyte Differential Counting\" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. \"Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop\", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., Chen, T., Orpilla, J. and Lee, J.H. \"A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry\", Tissue Antigens 50:197-201 (1997).

Additional Info

Reacts to HLA Class 2 Antigen-DR9. Additional crossreactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.