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HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)

Cat no: H6202-40A

HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)

Hmgb3 belongs to the high mobility group HMG) protein superfamily. It contains DNA-binding HMG box domains and is thought to play a fundamental role in DNA replication, nucleosome assembly and transcription. Hmgb3 is a sequence-independent chromatin-binding protein\nthat is preferentially expressed in hematopoietic stem cells. It can alter DNA structure to facilitate transcription factor binding. Enforced expression of Hmgb3 inhibits B-cell and\nmyeloid differentiation and thus, down-regulation of Hmgb3 protein levels has been concluded to be an important step for myeloid and B-cell differentiation. Hmgb3 deficiency\npossibly leads to a failure of hematopoietic stem cells to expand into normal numbers of common lymphoid progenitors and common myeloid progenitors. Hmgb3 progenitors attain the ability to expand rapidly and differentiate into normal numbers of hematopoietic cells. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:64000\nWestern Blot: 0.01-0.03ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman Lung lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H6202-40A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by antigen immunoaffinity chromatography.

Isotype

IgG

References

1. Yu F, et al, let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell. 2007 Dec 14;131(6):1109-23. PMID: 18083101.

Additional Info

Recognizes human HMG4 at ~24kD. Species sequence homology: mouse, bovine.

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