HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a, High Mobility Group Box 3, HMGB3, MGC90319, Nonhistone Chromosomal Protein HMG4)

HMGB3, also known as High mobility group protein B3, is a DNA associated protein that binds preferentially single-stranded DNA and unwinds double stranded DNA. This transcription factor belongs to the HMGB family and high mobility group (HMG) protein superfamily and contains two HMG box DNA-binding domains. Like other members of the family, HMGB3 plays a fundamental role in DNA replication, nucleosome assembly and transcription. Other functions include regulation of balance between hematopoietic stem cell self-renewal and differentiation and may also be required during embryonic development. HMGB3 is predominantly expressed in placenta.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 3-5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse placenta\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H6202-41A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Wilke, K. et al. Gene. 198:269-274 (1997). 2. Vaccari, T. et al. Genomics. 49:247-252 (1998). 3. Nemeth, MJ. et al. Blood. 102:1298-1306 (2003). 4. Nemeth, MJ. et al. Proc. Nat. Acad. Sci. 103:13783-13788 (2006).

Additional Info

Recognizes human HMGB3. Species Crossreactivity: bovine, equine, mouse, opossum, rat. Species sequence homology: chicken, xenopus, zebrafish.