The Acetylcholinesterase (AChE) Activity kit is designed to quantitatively measure AChE activity in a variety of samples. A human AChE standard is provided to generate a standard curve for the assay. The kit utilizes a proprietary non-fluorescent molecule, that covalently binds to the thiol product of the reaction between the AChE Substrate and AChE in the standards or samples, yielding a fluorescent product read at 510 nm in a fluorescent plate reader with excitation at 390 nm.