Human Factor B (FB) ELISA kit is designed for detection of human factor B in plasma, serum, saliva, urine, milk and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Factor B in less than 4 hours. An antibody specific for Factor B has been pre-coated onto a 96-well microplate with removable strips. Factor B in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for Factor B, which is recognized by a streptavidinperoxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
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