Purified HSV-2 antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the HSV-2 IgG-specific antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of HSV-2 IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.