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HXK I / HK1 mAb

Cat no: MB0140

HXK I / HK1 mAb

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

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SPECIFICATIONS

Catalog Number

MB0140

Size

100ug/100ul

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

liquid

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Recombinant full length Human HXK I.

Species

This antibody detects endogenous levels of Hexokinase-1 and does not cross-react with related proteins

Dilutions

WB: 1:1000~10000\nICC: 1:100~500

Swiss Prot

P19367

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

Predicted band size:102kDa \nObserved band size:102kDa

Alternative Names

HK I,glycolytic enzyme,hexokinase type I brain form hexokinase; HKI; HXK1; HK1-ta; HK1-tb; HK1-tc; Hexokinase-1; Hexokinase 1; Hexokinase1

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