IKK(beta) (L193) pAb

The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase (alpha) (IKK(alpha)), previously designated CHUK, interacts with IkB-(alpha) and specifically phosphorylates IkB-(alpha) on Serines 32 and 36, the sites that trigger its degradation. IKK(alpha) appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK(alpha) is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK(alpha), IKK(beta) and IKK(gamma) (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

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SPECIFICATIONS

Catalog Number

BS1540

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 171-220 of Human IKK(beta).

Species

IKK-(beta) (L193) pAb detects endogenous levels of IKK-(beta) protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

O14920

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 86 kDa

Alternative Names

Inhibitor of nuclear factor kappa B kinase beta subunit; I-kappa-B-kinase beta; IkBKB; IKK-B; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB