Il16, Recombinant, Mouse, aa22-287, His-tag (Pro-interleukin-16, Interleukin-16, IL-16, Lymphocyte Chemoattractant Factor, LCF)

IL-16 was initially identified as a chemotactic cytokine, but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases. The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20kD active form that binds to and signals through CD4. Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders. At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus. This recombinant protein fragment is derrived from this longer isoform.\n\nSource:\nRecombinant protein corresponding to mouse IL-16, fused to His-tag at C-terminal expressed in E.coli.\n\nMolecular Weight:\n~29kD\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

149786

Size

50ug

Form

Supplied as a liquid in 20mM Tris, 0.5M sodium chloride, 250mM Imidazole, 5mM beta-mercaptoethanol, 10% glycerol.

Purity

~95%

References

1. Cruikshank WW, Center DM, Nisar N, et al. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD expression. Proc. Natl. Acad. Sci. USA 1994; 91:5109-13. 2. Interleukin-16. Cruikshank WW, Kornfeld H, and Center DM. J. Leukoc. Biol. 2000; 67:757-66. 3. Zhang Y, Center DM, Wu DM, et al. Processing and activation of pro-interleukin-16 by caspase-3. J. Biol. Chem. 1998; 273:1144-9. 4. Maciaszek JW, Parada NA, Cruikshank WW, et al. IL-16 represses HIV-1 promoter activity. J. Immunol. 1997; 158:5-8.